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Corneal Epithelial Cell Growth Kit
Corneal Epithelial Cell Growth Kit
規(guī)格:
貨期:
編號:B240695
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板 



  
    

      產品名稱
      Corneal Epithelial Cell Growth Kit
    

    

      商品貨號
      B240695
    

      

      Biosafety Level
      1 




Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

    

      

      Product Format
      frozen 1 kit
    

      

      Storage Conditions
      -20°C (or -70°C for long-term storage)
    

      

      Comments
      
Corneal Epithelial  Cell Growth Kit (ATCC PCS-700-040) contains components that when added to Corneal Epithelial Cell Basal Medium (ATCC PCS-700-030) creates a complete ATCC® Primary Cell Solution&trad; culture environment for epithelial cells derived from normal human corneal. The serum-free medium formulation is designed to support normal corneal epithelial cell morphology as well as promote rapid growth and proliferation. No feeder layers, extracellular matrix proteins or other substrates are required.



The final concentration for each component in ATCC complete corneal epithelial growth medium is as follows:


    
    
  • Apo-Transferrin: 5 µg/mL
    • 
    
  • Epinephrine: 1.0 µM
    • 
    
  • Extract P: 0.4%
    • 
    
  • Hydrocortisone: 100 ng/mL
    • 
    
  • L-Glutamine: 6 mM
    • 
    
  • rh Insulin: 5 µg/ml
    • 
    
  • CE Growth Factor: Proprietary formulation
    • 




    

      

      Complete Growth Medium
      
    
    
  1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight. 
    2. 
    
  2. Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates prior to adding to the basal medium. 
    3. 
    
  3. Obtain one bottle of Corneal Epithelial Cell Basal Medium (485 mL) from cold storage.
    4. 
    
  4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying with 70% ethanol.
    5. 
    
  5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component to the bottle of basal medium using a separate sterile pipette for each.
    6. 
    
    Table 1. Corneal Epithelial Cell Growth Kit Components  
    
    
    
    
    
        
            
    
                 
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    Component                      
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
     
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    		
    
    
      
    
      Volume
      
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    Volume
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
     
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    		
    
    
      
    
      Sterility Tests
      
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    Final Concentration
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
     
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    		
    
    
      
    
      Functional Tests
      
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    Apo-transferrin
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
     
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    		
    
    
      
    
      C of A
      
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    0.5 mL
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
     
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    		
    
    
        

    

						
					
					
    
							
    
							  
							  
							    
    
							      梅經理
							      17280875617
							      1438578920
							    
    
							    
    
							      胡經理
							      13345964880
							      2438244627
							    
    
							    
    
							      周經理
							      17757487661
							      1296385441
							    
    
							    
    
							      于經理
							      18067160830
							      2088210172
							    
    
							    
    
							      沈經理
							      19548299266
							      2662369050
							    
    
							    
    
							      李經理
							      13626845108
							      972239479
							    
    
							    
							      
							
    
					
    
				
				
    
					
				
    
			
			
		
		
    
	
  
    
	









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